Sunday, June 30, 2013

Philly rapper ordered to take etiquette classes

(AP) ? A judge has ordered rapper Meek Mill to attend etiquette classes and notify his probation officer before he takes any trips outside of the commonwealth.

Common Pleas Court Judge Genece Brinkley on Friday told the rapper, whose real name is Robert Williams, he must complete the classes before Aug. 4, The Philadelphia Inquirer (http://bit.ly/15REv46) reported.

The orders came at a probation violation hearing for Williams, who is on probation for a 2008 gun and drug conviction for which he was sentenced to 11 to 23 months in prison. He served eight months in jail and began five years of probation in the fall of 2009.

Assistant District Attorney Noel Ann DeSantis said Williams' statements on Twitter and other social media had been followed by threats to his probation officer from some of his fans.

Williams told the judge at the contentious hearing that detailing his travel plans was difficult because many of his business activities are arranged on short notice.

"I have my own record label with seven artists. ... I do radio. I do interviews," he said.

The judge said Williams needed etiquette classes to refine his use of social media and to help him explain the nature of his business to the court, adding that the etiquette classes were "more important than any concerts he might have."

Brinkley in December barred the rapper from touring for a month after finding that he violated probation restrictions. Williams' attorney argued at the time that the restrictions were preventing his client from earning a living, and said Williams didn't need to check in with his probation officer because his fans frequently take pictures of him when he's touring.

Williams' "Dreams & Nightmares" album debuted in October and he appeared in Jay-Z's Made In America festival earlier this year.

___

Information from: The Philadelphia Inquirer, http://www.philly.com

Source: http://hosted2.ap.org/APDEFAULT/4e67281c3f754d0696fbfdee0f3f1469/Article_2013-06-29-People-Meek%20Mill/id-1f51b52d533d4ca7af339f19767d10f1

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City and colour rocks Canada Day festivities in NOTL

NIAGARA-ON-THE-LAKE?-?

Canada Day festivities get underway in Niagara-on-the-Lake as Parks Canada hosts its second annual outdoor concert at Butler's Barracks.

Doors opened at 2:30 and the concert line-up includes Niagara's own City and Colour, otherwise known as Dallas Green.

This is Green's first performance as City and Colour in Niagara since the Alexisonfire singer released his first solo album seven years ago.

Follow The Standard's reporting team of Marlene Bergsma and Julie Jocsak at they tweet from the event. Add your comments and photos with #RockNOTL

Last year's Tragically Hip concert attracted 17,000 people and raised $65,000 for Parks Canada.

The set list is as follows:

  • Yukon Blonde 3:15
  • Serena Ryder 4:30
  • Jimmy Eat World 5:45
  • Metric 7:15
  • City and Colour 9:00

Source: http://www.niagarafallsreview.ca/2013/06/29/city-and-colour-rocks-canada-day-festivities-in-notl

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Captain Planet and the Planeteers Film Being Optioned by Sony

30
Jun
2013

Captain Planet

Sony Pictures is looking at optioning the rights to the 1990?s animated series Captain Planet and the Planeteers.

I?ve said it before, but now I am truly convinced that Hollywood is completely out of ideas. While I am an unabashed fan of cartoons and watch them to this day, Captain Planet never caught my interest. I gave it a try, and while I?ve lived through cartoons such as Scooby-Doo Meets the Harlem Globetrotters, this series was just too far down the ladder for even me to watch.

That being said, for some?unknown?reason this 1900-1992 series is in play again for making a comeback. According to The Hollywood Reporter, Sony Pictures is in negotiations to acquire the rights, and making it even more mind boggling, it may be live-action.

Even if this option goes through, there is no guarantee it will see the light of day. There has already been an attempt at a film, but it never got past the script stage. And there has also been an attempt at a live-action TV series that never came?together.

If you are unfamiliar with the series ? and you should be grateful if you are ? five teenagers from around the world (North America, Africa, Eastern Europe, Asia and South America) are granted powers by Gaia, the Earth mother to protect the planet?s ecological system. When they come up against something they can?t overcome, they could combine their powers and summon Captain Planet.

While the heroes did take on villains such as?Looten Plunder, the series was just all about hitting you over the head with the ecological message. It was just? bad.

With this and Trolls in the work, you have to wonder if Hollywood has just given up.

[ Source The Hollywood Reporter ]

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Pakistan Christian girl accused of blasphemy moves to Canada over security fears

  • A supporter of Pakistani cleric Tahir-ul Qadri poses with a victory sign at a protest rally in Islamabad on January 17, 2013. Pakistani ministers held talks with a cleric leading a mass protest in Islamabad in an attempt to avert a political crisis and end a demonstration that has heaped pressure on the fragile government. (ASIF HASSAN/AFP/Getty Images)

  • A supporter of Pakistani cleric Tahir-ul Qadri waves the national flag at a protest rally in Islamabad on January 17, 2013. Pakistani ministers held talks with a cleric leading a mass protest in Islamabad in an attempt to avert a political crisis and end a demonstration that has heaped pressure on the fragile government. (ASIF HASSAN/AFP/Getty Images)

  • Supporters of Pakistani cleric Tahir-ul Qadri gather at a protest rally in Islamabad on January 17, 2013. Pakistani ministers held talks with a cleric leading a mass protest in Islamabad in an attempt to avert a political crisis and end a demonstration that has heaped pressure on the fragile government. (ASIF HASSAN/AFP/Getty Images)

  • Pakistani cleric Tahir-ul Qadri addresses his supporters from his makeshift room at a protest rally in Islamabad on January 17, 2013. Pakistani ministers held talks with a cleric leading a mass protest in Islamabad in an attempt to avert a political crisis and end a demonstration that has heaped pressure on the fragile government. (ASIF HASSAN/AFP/Getty Images)

  • Supporters of Pakistani cleric Tahir-ul Qadri chant slogans at a protest rally in Islamabad on January 17, 2013. Pakistani ministers held talks with a cleric leading a mass protest in Islamabad in an attempt to avert a political crisis and end a demonstration that has heaped pressure on the fragile government. (ASIF HASSAN/AFP/Getty Images)

  • Supporters of Pakistani cleric Tahir-ul Qadri chant slogans at a protest rally in Islamabad on January 17, 2013. Pakistani ministers held talks with a cleric leading a mass protest in Islamabad in an attempt to avert a political crisis and end a demonstration that has heaped pressure on the fragile government. (ASIF HASSAN/AFP/Getty Images)

  • A supporter of Pakistani cleric Tahir-ul Qadri holds a placard at a protest rally in Islamabad on January 17, 2013. Pakistani ministers held talks with a cleric leading a mass protest in Islamabad in an attempt to avert a political crisis and end a demonstration that has heaped pressure on the fragile government. (ASIF HASSAN/AFP/Getty Images)

  • Supporters of Pakistani cleric Tahir-ul Qadri gather inthe rain at a protest rally in Islamabad on January 17, 2013. Pakistani ministers held talks with a cleric leading a mass protest in Islamabad in an attempt to avert a political crisis and end a demonstration that has heaped pressure on the fragile government. (ASIF HASSAN/AFP/Getty Images)

  • Supporters of Pakistani cleric Tahir-ul Qadri gather at a protest rally in Islamabad on January 17, 2013. Pakistani ministers held talks with a cleric leading a mass protest in Islamabad in an attempt to avert a political crisis and end a demonstration that has heaped pressure on the fragile government. (ASIF HASSAN/AFP/Getty Images)

  • Supporters of Pakistani moderate preacher Tahir-ul Qadri gather on the fourth day of a protest rally in Islamabad on January 17, 2013. Pakistan's President intervened to stop authorities from using force against protesters who are calling for parliament to be dissolved in Islamabad's largest political rally in years. (ASIF HASSAN/AFP/Getty Images)

  • A supporter of Pakistani moderate preacher Tahir-ul Qadri waves the national flag on the fourth day of a protest rally in Islamabad on January 17, 2013. Pakistan's President intervened to stop authorities from using force against protesters who are calling for parliament to be dissolved in Islamabad's largest political rally in years. (ASIF HASSAN/AFP/Getty Images)

  • Supporters of Pakistani cleric Tahir-ul Qadri gesture the victory symbol during a protest rally in Islamabad on January 17, 2013. A populist Pakistani cleric calling for electoral reforms announced that a mass sit-in of tens of thousands of people camped outside parliament in Islamabad would end January 17. (FAROOQ NAEEM/AFP/Getty Images)

  • Supporters of Pakistani cleric Tahir-ul Qadri gather in the rain during a protest rally in Islamabad on January 17, 2013. A populist Pakistani cleric calling for electoral reforms announced that a mass sit-in of tens of thousands of people camped outside parliament in Islamabad would end January 17. (FAROOQ NAEEM/AFP/Getty Images)

  • A supporter of Pakistani cleric Tahir-ul Qadri wears a plastic bag in the rain during a protest rally in Islamabad on January 17, 2013. A populist Pakistani cleric calling for electoral reforms announced that a mass sit-in of tens of thousands of people camped outside parliament in Islamabad would end January 17. (FAROOQ NAEEM/AFP/Getty Images)

  • A supporter of Pakistani cleric Tahir-ul Qadri enjoys the rain at a protest rally in Islamabad on January 17, 2013. A populist Pakistani cleric calling for electoral reforms announced that a mass sit-in of tens of thousands of people camped outside parliament in Islamabad would end January 17. (FAROOQ NAEEM/AFP/Getty Images)

  • Female supporters of moderate preacher Tahir-ul Qadri stand guard to protect sleeping women taking part in the fourth day of protests in Islamabad early on January 17, 2013. Pakistan's president on January 16 intervened to stop authorities from using force against protesters who are calling for parliament to be dissolved in Islamabad's largest political rally in years. (ASIF HASSAN/AFP/Getty Images)

  • Supporters of Pakistani cleric Tahir-ul Qadri rest on the third day of the protest rally in Islamabad on January 16, 2013. A populist cleric Wednesday urged Pakistani politicians to join tens of thousands taking part in the largest protest in Islamabad for years, ratcheting up the pressure on the government to step down. (ASIF HASSAN/AFP/Getty Images)

  • Pakistani students, civil society and Pakistan Muslim League Nawaz party activists light candles in the favor of democracy in Lahore on January 16, 2013. Pakistan's main opposition leader Nawaz Sharif demanded January 16, that the government immediately announce a timetable for elections. (Arif Ali/AFP/Getty Images)

  • A supporter of Pakistani cleric Tahir-ul Qadri dances to drums on the third day of the protest rally in Islamabad on January 16, 2013. A populist cleric Wednesday urged Pakistani politicians to join tens of thousands taking part in the largest protest in Islamabad for years, ratcheting up the pressure on the government to step down. (ASIF HASSAN/AFP/Getty Images)

  • Activists of the ruling Pakistan Peoples Party (PPP) burn tyres on a street at a protest rally in Lahore on January 16, 2013, against the Supreme Court order to arrest of the prime minister Raja Pervez Ashraf. Pakistan's top judge January 15, ordered the arrest of the prime minister over graft allegations, threatening to worsen turmoil as thousands of protesters demanded the government step down. (Arif Ali/AFP/Getty Images)

  • A Pakistani villager from the northwest mourns the death of a relative during a protest in the provincial capital Peshawar on January 16, 2013. Demonstrators said gunmen wearing military uniforms stormed homes in Bara Tehsil in Khyber Agency, some 30 kilometers from Peshawar and shot 18 villagers dead in an overnight raid. (A Majeed/AFP/Getty Images)

  • Supporters of moderate preacher Tahir-ul Qadri rest on the third day of a protest rally in Islamabad on January 16, 2013. Pakistani protesters rallied for a third day January 16 in the largest political demonstration seen for years in the capital, calling on the government to quit after the Supreme Court ordered the arrest of the prime minister. (ASIF HASSAN/AFP/Getty Images)

  • Supporters of moderate preacher Tahir-ul Qadri prepare breakfast at dawn on the third day of a protest rally in Islamabad on January 16, 2013. Pakistani protesters rallied for a third day January 16 in the largest political demonstration seen for years in the capital, calling on the government to quit after the Supreme Court ordered the arrest of the prime minister. (ASIF HASSAN/AFP/Getty Images)

  • Pakistani Frionter Constables (FC) and policemen stand guard over a barricade of shipping containers on Constitution avenue on the third day of a protest rally in Islamabad on January 16, 2013. Pakistani protesters rallied for a third day January 16 in the largest political demonstration seen for years in the capital, calling on the government to quit after the Supreme Court ordered the arrest of the prime minister. (ASIF HASSAN/AFP/Getty Images)

  • A supporter of moderate preacher Tahir-ul Qadri waves this Pakistani national flag on the third day of a protest rally in Islamabad on January 16, 2013. An estimated 25,000 to 50,000 people have poured into Islamabad from across the country, devoted followers of moderate preacher Tahir-ul Qadri who is calling for the government to step down and radical reforms. It is the largest protest in the capital since the Pakistan People's Party won elections in 2008, ending a decade of military rule and forming what in March will be the country's first civilian government to complete a term in office. (ASIF HASSAN/AFP/Getty Images)

  • Supporters of moderate preacher Tahir-ul Qadri gather on the third day of a protest rally in Islamabad on January 16, 2013. An estimated 25,000 to 50,000 people have poured into Islamabad from across the country, devoted followers of moderate preacher Tahir-ul Qadri who is calling for the government to step down and radical reforms. It is the largest protest in the capital since the Pakistan People's Party won elections in 2008, ending a decade of military rule and forming what in March will be the country's first civilian government to complete a term in office. (ASIF HASSAN/AFP/Getty Images)

  • Source: http://www.huffingtonpost.ca/2013/06/30/pakistan-christian-girl-a_n_3524563.html?utm_hp_ref=canada-politics

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    SmackDown live results: June 28, 2013

    All WWE programming, talent names, images, likenesses, slogans, wrestling moves, trademarks, logos and copyrights are the exclusive property of WWE, Inc. and its subsidiaries. All other trademarks, logos and copyrights are the property of their respective owners. ? 2013 WWE, Inc. All Rights Reserved. This website is based in the United States. By submitting personal information to this website you consent to your information being maintained in the U.S., subject to applicable U.S. laws. U.S. law may be different than the law of your home country. WrestleMania XXIX (NY/NJ) logo TM & ? 2013 WWE. All Rights Reserved. The Empire State Building design is a registered trademark and used with permission by ESBC.

    Source: http://www.wwe.com/shows/smackdown/2013-06-28/smackdown-live-results-june-28-2013

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    Mumfords to close Glastonbury after Stones triumph

    AAA??Jun. 30, 2013?6:19 AM ET
    Mumfords to close Glastonbury after Stones triumph
    AP

    Mick Jagger, center, Ronnie Wood, left ,Charlie Watts, rear on drums and Keith Richards, right, of British band the Rolling Stones, perform on the Pyramid main stage at Glastonbury, England, Saturday, June 29, 2013. Thousands of music fans have arrived for the festival to see headliners, Arctic Monkeys, Mumford and Sons and the Rolling Stones.(Photo by Joel Ryan/Invision/AP)

    Mick Jagger, center, Ronnie Wood, left ,Charlie Watts, rear on drums and Keith Richards, right, of British band the Rolling Stones, perform on the Pyramid main stage at Glastonbury, England, Saturday, June 29, 2013. Thousands of music fans have arrived for the festival to see headliners, Arctic Monkeys, Mumford and Sons and the Rolling Stones.(Photo by Joel Ryan/Invision/AP)

    Mick Jagger of British band the Rolling Stones performs on the Pyramid main stage at Glastonbury, England, Saturday, June 29, 2013. Thousands of music fans have arrived for the festival to see headliners, Arctic Monkeys, Mumford and Sons and the Rolling Stones.(Photo by Joel Ryan/Invision/AP)

    The crowd watches British band the Rolling Stones perform on the Pyramid main stage at Glastonbury, England, Saturday, June 29, 2013. Thousands of music fans have arrived for the festival to see headliners, Arctic Monkeys, Mumford and Sons and the Rolling Stones.(Photo by Joel Ryan/Invision/AP)

    Mick Jagger of British band the Rolling Stones performs on the Pyramid main stage at Glastonbury, England, Saturday, June 29, 2013. Thousands of music fans have arrived for the festival to see headliners, Arctic Monkeys, Mumford and Sons and the Rolling Stones.(Photo by Joel Ryan/Invision/AP)

    Mick Jagger, center, Ronnie Wood, left, and Mick Jones of British band the Rolling Stones perform on the Pyramid main stage at Glastonbury, England, Saturday, June 29, 2013. Thousands of music fans have arrived for the festival to see headliners, Arctic Monkeys, Mumford and Sons and the Rolling Stones.(Photo by Joel Ryan/Invision/AP)

    (AP) ? The Glastonbury Festival is wrapping up, with many fans still on a high from the Rolling Stones' first-ever gig at Britain's leading music extravaganza.

    Festival founder Michael Eavis declared the concert "the high spot of 43 years of Glastonbury."

    The Stones played for more than two hours Saturday, giving fans a clutch of hits, from opener "Jumpin' Jack Flash" through to encores of "You Can't Always Get What You Want" and "Satisfaction."

    Organizers estimate 100,000 people watched the show, including celebrity music fans Prince Harry and Kate Moss.

    The gig was a coup for the festival, although there were grumbles from TV viewers because the Stones agreed to let the BBC air only an hour of its set.

    The festival ends Sunday with a performance by Mumford & Sons.

    Associated Press

    Source: http://hosted2.ap.org/APDEFAULT/4e67281c3f754d0696fbfdee0f3f1469/Article_2013-06-30-Britain-Glastonbury/id-0f85c1a05d0446cba9854a926ffe5ad6

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    Top 3 marketing approach to boost your online traffic - Web-Hosting ...

    The most common way to drive traffic to a website is to properly promote it in different ways. There are many strategies that were developed but only few are remaining that are very much effective and useful to the website.
    In the new form of marketing, the competition in every industry has become very complex and unstable due to the continuous changes of search engines on which methods are going to benefit the website in the long run that will not cause penalization.

    However, despite the unstable methods of marketing a business or website online, there are still effective approach that have never been ignored by many internet marketers to help and make their business succeed and standout in their market niche and these includes:

    SEO - Search Engine Optimization is very popular and a must have strategy online in promoting a website. The future of your website performance in organic search results or SERPs will depend on how competitive and effective the SEO approach you are implementing as this can have a negative result on your website which could last for months.?

    The demand of SEO is very popular for many companies as SEO has become the most efficient in marketing a website that can have good results in the long run in just minimal expenses and efforts needed. There are guidelines that you should follow given by search engines for better search rankings and performance of your website.

    SEM - Contradict to SEO which is more of organic and free, Search Engine Marketing has become widely known and accepted by many internet marketers and business owners even this method is a form of online advertisements such as: CPC, PPC and CPA. The effectiveness of this approach is definitely beneficial as each visitor coming from online contextual ad is a valid and potential prospect to a website. Unlike in SEO, not all visitors coming from organic search will be converted into clients. However, this method is just quite expensive as this may require a monthly payment to keep your contextual ads remain live and active

    SMM - Online marketing does not only cover search engines to generate and drive traffic to a website. Since social media was introduced as an online marketing tool, this has widened the scope of online marketing. The success of social media in establishing online communication was embraced by many marketers to develop and build a strong business. Also, social media platforms like FB, G+, Twitter and LinkedIn can be used as a medium by owners and clients to have a direct contact with each other which is a good start to develop a strong online relationship that could strengthen the business's online presence.

    Email Marketing - This method is not quite new to many internet savvy as this is very widely used by many marketers online. However, this will not guarantee the success of business if the online methods that they are using to keep their business grow is email marketing alone may not be enough. The success in email marketing is actually depends on how many contacts you have and how are they related to your industry. If your contacts are not related to your industry, there is no guarantee that you will benefit from them and all your effort could be wasted. So it?s only important that you seek out for only your target audience to avoid being lost in the middle of nowhere.

    In a world where everything has become very easy not only for many individuals but also for many companies, online marketing is the best way and convenient for small and huge businesses to marketing their services and increase the opportunity for their business, it is really important that we take advantage of it and use it to success in our industry.

    Source: http://www.web-hosting-reviews.info/2013/06/boost-online-traffic.html

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    Mimicking living cells: Synthesizing ribosomes

    June 29, 2013 ? Synthetic biology researchers at Northwestern University, working with partners at Harvard Medical School, have for the first time synthesized ribosomes -- cell structures responsible for generating all proteins and enzymes in our bodies -- from scratch in a test tube.

    Others have previously tried to synthesize ribosomes from their constituent parts, but the efforts have yielded poorly functional ribosomes under conditions that do not replicate the environment of a living cell. In addition, attempts to combine ribosome synthesis and assembly in a single process have failed for decades.

    Michael C. Jewett, a synthetic biologist at Northwestern, George M. Church, a geneticist at Harvard Medical School, and colleagues recently took another approach: they mimicked the natural synthesis of a ribosome, allowing natural enzymes of a cell to help facilitate the human-made construction.

    The technology could lead to the discovery of new antibiotics targeting ribosome assembly; an advanced understanding of how ribosomes form and function; and the creation of tailor-made ribosomes to produce new proteins with exotic functions that would be difficult, if not impossible, to make in living organisms.

    "We can mimic nature and create ribosomes the way nature has evolved to do it, where all the processes are co-activated at the same time," said Jewett, who led the research along with Church. "Our approach is a one-pot synthesis scheme in which we toss genes encoding ribosomal RNA, natural ribosomal proteins, and additional enzymes of an E. coli cell together in a test tube, and this leads to the construction of a ribosome."

    Jewett is an assistant professor of chemical and biological engineering at Northwestern's McCormick School of Engineering and Applied Science.

    The in vitro construction of ribosomes, as demonstrated in this study, is of great interest to the synthetic biology field, which seeks to transform the ability to engineer new or novel life forms and biocatalytic ensembles for useful purposes.

    The findings of the four-year research project were published June 25 in the journal Molecular Systems Biology.

    Comprising 57 parts -- three strands of ribonucleic acid (RNA) and 54 proteins -- ribosomes carry out the translation of messenger RNA into proteins, a core process of the cell. The thousands of proteins per cell, in turn, carry out a vast array of functions, from digestion to the creation of antibodies. Cells require ribosomes to live.

    Jewett likens a ribosome to a chef. The ribosome takes the recipe, encoded in DNA, and makes the meal, or a protein. "We want to make brand new chefs, or ribosomes," Jewett said. "Then we can alter ribosomes to do new things for us."

    "The ability to make ribosomes in vitro in a process that mimics the way biology does it opens new avenues for the study of ribosome synthesis and assembly, enabling us to better understand and possibly control the translation process," he said. "Our technology also may enable us in the future to rapidly engineer modified ribosomes with new behaviors and functions, a potentially significant advance for the synthetic biology field."

    The synthesis process developed by Jewett and Church -- termed "integrated synthesis, assembly and translation" (iSAT) technology -- mimics nature by enabling ribosome synthesis, assembly and function in a single reaction and in the same compartment.

    Working with E. coli cells, the researchers combined natural ribosomal proteins with synthetically made ribosomal RNA, which self-assembled in vitro to create semi-synthetic, functional ribosomes.

    They confirmed the ribosomes were active by assessing their ability to carry out translation of luciferase, the protein responsible for allowing a firefly to glow. The researchers then showed the ability of iSAT to make a modified ribosome with a point mutation that mediates resistance to the antibiotic clindamycin.

    The researchers next want to synthesize all 57 ribosome parts, including the 54 proteins.

    "I'm really excited about where we are," Jewett said. "This study is an important step along the way to synthesizing a complete ribosome. We will continue to push this work forward."

    Jewett and Church, a professor of genetics at Harvard Medical School, are authors of the paper, titled "In Vitro Integration of Ribosomal RNA Synthesis, Ribosome Assembly, and Translation." Other authors are Brian R. Fritz and Laura E. Timmerman, graduate students in chemical and biological engineering at Northwestern.

    The work was carried out at both Northwestern University and Harvard Medical School.

    Source: http://feeds.sciencedaily.com/~r/sciencedaily/most_popular/~3/_1dSF3gpNfo/130629164739.htm

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    Saturday, June 29, 2013

    It s All Elementary: Decades of Insights from Nobel Laureates in Chemistry (preview)

    Cover Image: July 2013 Scientific American MagazineSee Inside

    As laureates and newcomers in chemistry form new bonds, we celebrate their achievements, past and future


    Image: BOMBOLAND

    In Brief

    • Every summer in Lindau, Germany, Nobel Prize recipients and up-and-coming researchers in a particular field gather and socialize. This year the meeting focuses on chemistry.
    • In celebration of the Lindau meeting, Scientific American has chosen 11 excerpts from articles in the magazine's archives authored by Nobel Laureates in chemistry.
    • The excerpts cover a surprisingly wide range of subjects, from the details of atoms and molecules to the chemical makeup of the earth's atmosphere. Some describe fundamental discoveries about natural elements; others recount researchers' attempts to create brand-new substances.
    • Some of the questions that preoccupied chemists many decades ago remain unanswered today, and certain man-made materials once considered unusual and of uncertain value have now become commonplace.

    Chemists typically concern themselves with the properties of matter at the level of atoms and molecules. That focus may seem narrow, but it is quite the opposite. Chemistry reveals a great deal about the world around us, including the origins of life, how the human body works and how tiny molecules can profoundly change the earth's atmosphere. And, of course, chemistry makes it possible to create useful materials not found in nature.

    Such insights have been celebrated for more than a century, as evidenced by the long record of Nobel Prizes for advances in chemistry. This summer past winners of the prize are joining up-and-coming scientists in Lindau, Germany, to discuss previous breakthroughs and future prospects. In honor of the event?the 63rd Lindau Nobel Laureate Meeting?Scientific American is publishing excerpts from articles authored by Nobel Laureates in chemistry over the years, beginning on page 70. Many of the snippets resonate with researchers' priorities today.

    This article was originally published with the title A Nobel Gathering.

    Source: http://rss.sciam.com/~r/sciam/chemistry/~3/NwkrPmZ5Nm8/article.cfm

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    Lessons from the Tax Court: Create your own space ? Business ...

    Do you operate a business from home? You?re entitled to home office deductions only if you regularly and exclusively use the place as your principal place of business or where you meet or deal with customers, clients or patients in the normal course of business. Taxpayers are often tripped up by the requirement for ?exclusive? use.

    New case: An attorney in Georgia used the first floor of his house for his law practice before moving to an apartment. He then claimed deductions based on the entire first floor of the house plus two of the eight rooms in the apartment (a ?computer room? and a ?sun room?).

    The first floor of the house qualified for home office deductions. It was physically separated from the rest of the house and had a separate entrance and awning.

    But the attorney didn?t meet his ?burden of proof? for the apartment deductions. He did not offer evidence how the computer room was used nor did he substantiate his claim that the sun room was used to store documents and supplies. Thus, the deductions based on the two rooms in the apartment were denied. (Longino, TC Memo TC Memo 2013-80)

    Like what you've read? ...Republish it and share great business tips!

    Attention: Readers, Publishers, Editors, Bloggers, Media, Webmasters and more...

    We believe great content should be read and passed around. After all, knowledge IS power. And good business can become great with the right information at their fingertips. If you'd like to share any of the insightful articles on BusinessManagementDaily.com, you may republish or syndicate it without charge.

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    Source: http://www.businessmanagementdaily.com/35537/lessons-from-the-tax-court-create-your-own-space

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    Before purchasing this vehicle, it is your responsibility to address any and all differences between information on this website and the actual vehicle specifications and/or any warranties offered prior to the sale of this vehicle. Vehicle data on this website is compiled from publicly available sources believed by the publisher to be reliable. Vehicle data is subject to change without notice. The publisher assumes no responsibility for errors and/or omissions in this data the compilation of this data and makes no representations express or implied to any actual or prospective purchaser of the vehicle as to the condition of the vehicle, vehicle specifications, ownership, vehicle history, equipment/accessories, price or warranties.

    Source: http://www.verneideford.com/2011-Nissan-Sentra-Mitchell/vd/15555149

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    Perry, filibuster star clash over Texas abortions

    DALLAS (AP) ? A battle over proposed abortion restrictions in Texas became a personal grudge match Thursday between conservative Republican Gov. Rick Perry and a Democratic state senator whose lengthy, one-woman filibuster catapulted her to sudden, national political stardom.

    During a speech to the National Right to Life Conference, Perry singled out state Sen. Wendy Davis of Fort Worth, saying that her life story proves all children born into difficult circumstances deserve not be aborted.

    "She was the daughter of a single woman, she was a teenage mother herself. She managed to eventually graduate from Harvard Law School and serve in the Texas Senate," Perry said. "It's just unfortunate that she hasn't learned from her own example that every life must be given a chance to realize its full potential and that every life matters."

    In comments to reporters afterward, he went even further, saying that he was glad Davis' mother didn't chose to have an abortion.

    "What if her mom had said, 'I just can't do this. I don't want to do this,'" Perry said. "At that particular point in time I think it becomes very personal."

    Davis shot back in an email statement after Perry's speech: "Rick Perry's statement is without dignity and tarnishes the high office he holds."

    "They are small words that reflect a dark and negative point of view," she said. "Our governor should reflect our Texas values. Sadly, Gov. Perry fails that test."

    Davis starting working at 14 to help support a household of her single mother and three siblings. By 19, she was already married and divorced with a child of her own ? but she eventually graduated with honors from Harvard Law School and won her senate seat in an upset.

    On Tuesday, Davis' marathon speech and raucous outbursts from abortion rights protesters in the state Senate that kept lawmakers from approving sweeping restrictions that could make abortion all but impossible for many women in the second-largest state.

    Those efforts ran out the clock on the midnight deadline Tuesday to pass legislation during a special legislative session Perry called to tackle abortion and other key issues. But on Wednesday, he called a second, 30-day extra session and put tighter abortion rules at the top of the agenda he sets for lawmakers.

    The extra session has delayed Perry's expected announcement on whether he will seek a fourth full term as governor in elections next year. Davis is up for re-election then too, but some Democratic activists are urging her to seek the party's gubernatorial nomination.

    Source: http://news.yahoo.com/perry-filibuster-star-clash-over-texas-abortions-175240836.html

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    Facebook implements new policy to crack down on objectionable ad material

    Image

    No one really likes ads, but for better or worse, they're a sort of necessary evil when it comes to, you know, making money online. And while Facebook's not likely to stop sprinkling your stream with paid content, the social network announced a new plan today to tackle some of the more...questionable content that's made its way onto the site. Starting Monday, the service will implement a new review process for deciding which Pages and groups will get their own accompanying ads. That process will be manual to start, with an automated version in the future. Facebook plans to have all the offending violent, graphic and sexual content removed by the end of next week.

    Comments

    Source: Facebook Newsroom

    Source: http://www.engadget.com/2013/06/28/bye-bye-dirty-facebook-ads/?utm_medium=feed&utm_source=Feed_Classic&utm_campaign=Engadget

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    The Fourth of July Can Be a Dangerous Time For Pets

    HUMMELSTOWN, Pa., June 27, 2013 /PRNewswire-USNewswire/ -- Independence Day is one of the most celebrated times of the year and is fun for the whole family. Barbecues, parades, and fireworks are just a few of the ways many people like to celebrate with family and friends, but if your family includes pets, some of the most common sources of fun can be frightening or even dangerous.

    Backyard barbecues can potentially pose significant threats to pets. Never leave alcoholic drinks unattended or within reach of pets. Depending on the size of the pet, poisoning can occur quickly and can cause intoxication, weakness, depression, respiratory failure, and even coma. Also beware of all the picnic food. Many human foods ? even some fruits and vegetables ? can be toxic. Not only do you need to keep your pet out away from the buffet table, but make sure friends and neighbors aren't feeding them from their plates.

    Fireworks ? whether in the yard or at a community display ? can cause panic in pets, especially in a crowd. Even if you're just lighting a few in the backyard, make sure your pet is safely inside and not in an area where they can escape or run away. Likewise, keep matches and lighter fluid out of reach. Some matches contain damaging chlorates and lighter fluid is irritating to the skin and if swallowed.

    For more tips on Fourth of July safety, download our fact sheet at www.pavma.org.

    The Pennsylvania Veterinary Medical Association (PVMA) is the only statewide professional organization of over 2,200 veterinarians from across the Commonwealth. The association, which was established in 1883, strives to advance animal welfare and human health while ensuring the vitality of the veterinary profession. PVMA's website is available at www.pavma.org .

    SOURCE Pennsylvania Veterinary Medical Association (PVMA)

    Source: http://news.yahoo.com/fourth-july-dangerous-time-pets-192900086.html

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    From Egypt petition drive, a new grassroot wave

    An Egyptian man holds a banner with the word rebel in it during a demonstration supporting the Tamarod, Arabic for "rebel" campaign in Tahrir Square in Cairo, Egypt, Wednesday, June 26, 2013. In abstract terms, protests planned for Sunday, June 30, 2013 aiming to force out Egypt?s Islamist president violate a basic principle of democracy: If an election has been held, all must respect the results, otherwise it?s political chaos. Supporters of President Mohammed Morsi have been angrily making that argument for days. Those behind the protests insist he lost the legitimacy of that election victory by power grabs and missteps.(AP Photo/Manu Brabo)

    An Egyptian man holds a banner with the word rebel in it during a demonstration supporting the Tamarod, Arabic for "rebel" campaign in Tahrir Square in Cairo, Egypt, Wednesday, June 26, 2013. In abstract terms, protests planned for Sunday, June 30, 2013 aiming to force out Egypt?s Islamist president violate a basic principle of democracy: If an election has been held, all must respect the results, otherwise it?s political chaos. Supporters of President Mohammed Morsi have been angrily making that argument for days. Those behind the protests insist he lost the legitimacy of that election victory by power grabs and missteps.(AP Photo/Manu Brabo)

    Egyptians walk below a banner of Tamarod, Arabic for "rebel," with Arabic that reads, "tamarod, Egypt now is free," a campaign calling for the ouster of Egyptian President Mohammed Morsi, in Cairo, Egypt, Wednesday, June 26, 2013. Organizers of Tamarod had announced that they have collected millions of signatures supporting Morsi's ouster and early presidential elections. (AP Photo/Hassan Ammar)

    In this Sunday, June 2, 2013 photo, an Egyptian woman signs a petition for Tamarod, Arabic for "rebel", a campaign calling for the ouster of Egyptian President Mohammed Morsi and for early presidential elections in the Shubra neighborhood in Cairo, Egypt. Young activists are trying to rally public discontent with Egypt?s Islamist President Mohammed Morsi by fanning out in the streets and collecting millions of signatures on a petition calling for his removal. Morsi?s Muslim Brotherhood has dismissed the campaign as irrelevant, even illegal, but the signature drive has stirred up Egypt?s politics as the president nears the end of his tumultuous first year in office. (AP Photo/Hassan Ammar)

    FILE - In this Thursday, May 16, 2013 file photo, an Egyptian woman signs a leaflet for the campaign Tamarod, or "rebel" in Arabic, that seeks to withdraw confidence from Egypt's Islamist President Mohamed Morsi, in Cairo, Egypt. Opponents of Egypt?s Islamist president are convinced that nationwide protests planned for June 30 are their last opportunity to drive him from power. They say they have tapped into widespread public discontent over shortages, broken infrastructure, high prices and lack of security, and can bring that anger into the streets. (AP Photo/Nasser Nasser, File)

    FILE - In this Friday, May 17, 2013 file photo, An Egyptian activist covers her face with an applications for "Tamarod", Arabic for "rebel", a campaign calling for the ouster of Egyptian President Mohammed Morsi and for early presidential elections, during a protest in Tahrir Square, in Cairo, Egypt. Amid Egypt?s multiple woes under an Islamist-dominated administration, religion is not the political selling point it once was among Egyptians, one factor fueling planned weekend protests calling for Morsi?s fall. (AP Photo/Hassan Ammar, File)

    (AP) ? Teenager Gehad Mustafa wears an ultraconservative veil over her face and was raised in a family of staunch Muslim Brotherhood supporters. Yet for the past weeks, she has been walking though chaotic street markets and crowded subway stations, collecting signatures on a petition demanding Islamist President Mohammed Morsi step down.

    The months-long petition campaign by the group "Tamarod," Arabic for "rebel," is now culminating in nationwide protests Sunday in which the opposition hopes to bring out millions to force Morsi out of office, a year after his inauguration.

    But Tamarod's organizers say they are not stopping there. No matter what happens on Sunday, they say they have created through their petition drive a real grassroots network, an opposition version in the spirit of the Islamists' expert street organizing, and have brought forth a sort of second generation of street activists, like Mustafa, after the first that led the revolt against autocrat Hosni Mubarak in 2011.

    They want to use that network going ahead, to keep the public involved and to pressure the secular and liberal opposition parties, who the activists say have wasted opportunities through infighting and fragmentation, to get their act together.

    On a recent day, Tamarod's main office, steps away from Cairo's Tahrir Square, was bustling with several dozen volunteers as young as 13 and as old as their 50s and 60s. University professors, government employees, students and housewives sipped tea, smoked and chatted while going through the organization's prize possession: the sheaves of signed petitions still coming in from around the country, filling the office.

    The pages of signatures, they say, are proof of how deeply the country of 90 million has turned against the Muslim Brotherhood. They plan to announce their full count ahead of Sunday's protests but have claimed to have as many as 20 million signatures, which they collate, confirm and record in a database in a precise operation, knowing their count will be questioned.

    Among the volunteers was 17-year-old Mustafa. She said she turned against Morsi and his Muslim Brotherhood after the first protesters were killed under his administration in late 2012. "I saw the reality," she said. "You told us that the blood of the martyrs will not go in vain. But there were more ... falling under your rule."

    She joined Tamarod, which launched in late April, and volunteered to canvas the street for signatures. At one point, while passing out petitions in the subway, a man wearing the beard of a Muslim conservative attacked her, pulling the veil off her face. But other commuters then wrestled the man away in support of her.

    "This strengthened me. I felt what I am doing is right," she said.

    Organizers say Tamarod mushroomed across the country. Founded by five activists, its leadership is a central group of about 25, connected to a network of coordinators in Egypt's 27 provinces, each with a team of volunteers in towns and villages.

    The signatures are effectively a database of the dissatisfied: Each signatory puts his or her name, province of residence and national ID number.

    Collecting signatures in itself is a breakthrough, overcoming Egyptians' engrained resistance to signing onto any paper presented by a stranger, especially political, from the Mubarak days when doing so could get you a visit from state security or even arrested. Volunteers carrying the petitions brought politics into every corner ? weddings, slum alleys, buses and subways. Volunteers included strangers to political campaigning, from men selling cigarettes in kiosks to impoverished women selling in vegetable markets.

    Ahmed el-Masry, one of the founders of Tamarod, calls the success "astonishing."

    "I can't tell how many members out there. I can think that millions of Egyptians are members," he said.

    "At one point, people gave up (on Morsi) ... it reached a point where a new class of Brothers are gaining higher status in society that to join them, you have to let your beard grow. We reached a point where no one is heard but the president and his tribe."

    Brotherhood officials cast doubt on the signatures, claiming forgeries and multiple names. While Morsi says peaceful demonstrations are a legitimate form of expression, he and his allies also say Mubarak loyalists are behind the campaign and protests, trying to use the streets to topple an elected leader.

    A spokesman for the Brotherhood's Freedom and Justice Party said he sympathizes with some activists in Tamarod ? "the young revolutionaries who had great expectations out of the revolution. Due to their inexperience and age, they wanted to see change too fast and too soon and that is what I call frustration."

    But Abdel-Mawgoud el-Dardery said "opportunist politicians" are exploiting them for their political agenda and that former regime elements are exploiting both the politicians and the activists.

    "There is unholy alliance among these groups. They have insisted on having one enemy and that is President Morsi," he said.

    Tamarod activists say it is they who are leading the politicians of the mainly liberal and secular opposition parties and factions, trying to drag them into a better connection with the public. The campaign's plan calls for Morsi to leave, the chief justice of the Supreme Constitutional Court to become a largely symbolic interim president while a technocrat Cabinet governs, a panel would write a new constitution and presidential elections would be held in six months.

    Ahmed Abdu, one of the first Tamarod street campaigners, said the group will pressure the opposition to coalesce behind a candidate.

    If they can't get organized "we will pick one away from all the top leaders of opposition and we will be able to rally support to him."

    He blamed liberal parties for running multiple candidates in last year's presidential election, which resulted in a runoff between Morsi and a former Mubarak prime minister, forcing people to choose between an Islamist and a loyalist of the regime just ousted.

    "I hope they don't let us down again," Abdu said.

    Tamarod's nationwide network and pavement-pounding methods contrast with many of the political parties, which have struggled to establish a nationwide presence. That is in large part what opened the way for the Muslim Brotherhood, an 83-year-old organization that has highly disciplined cadres nationwide, and harder-line Islamist with their own organizations to dominate parliament elections in late 2011-early 2012, to ensure the constitution passed a December referendum, and to boost Morsi to victory.

    Tamarod's volunteers ? some former Morsi supporters, others who disliked him from the start ? had varying stories of what brought them to the campaign. Most said they were dismayed by what they call the Brotherhood's opportunism and determination to control the system rather than reform state institutions and police. That is a frequent refrain from critics of Morsi. His allies insist they are not trying to monopolize, that opponents have refused to work with them and that old regime loyalists have sabotaged their attempts at reform.

    At the Tamarod office, Doaa Mohammed, a young Justice Ministry employee, said the day after Morsi's election, a man on the street spit at her face and yelled, "Tomorrow, Morsi will get rid of you all."

    Mohammed wears a stylish scarf covering her hair, less strict than the more cloaking coverings and veils that hard-liners believe women should wear.

    She said managers in her ministry were replaced by Brotherhood sympathizers.

    "From day one, I have been treated like a second-class citizen. The Sister enjoys higher status than me just because she belongs to the group," she said, referring to the Muslim Sisters, the women's branch of the Brotherhood.

    The heart of Tamarod is its petitions. Through Facebook and Twitter, volunteers could download the form, copy it and distribute them among friends and family members or hit the streets for signatures, then get back in touch with coordinators to return the papers.

    At the Tamarod office, a psychology university lecturer-turned-volunteer explained how the papers are sorted by province, counted, scanned and entered into a database to ensure there are no doubled ID numbers and that the numbers ? which have prefixes by province ? match where they're said to come from. Much of the work takes place in a room labeled "Control Room. No Entry."

    Secrecy is tight. The university lecturer spoke on condition of anonymity ? he goes by the nickname "Maestro" ? so he could not be singled out for pressure by anyone trying to get to the petitions. He said only two of the founders know the whereabouts of the originals of the signed forms and are responsible for moving them every few days to new locations.

    "We are working in the daylight but they don't want us to work in the daylight," he said and added, "we are holding a pen and a paper. This is our weapon. And this is how we tell them, Enough"

    Associated Press

    Source: http://hosted2.ap.org/APDEFAULT/cae69a7523db45408eeb2b3a98c0c9c5/Article_2013-06-28-Egypt-New%20Grassroots/id-05e0c0fa9a6f4cba8b88f95927d29034

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    Matthew Morrison Engaged To Model Renee Puente (Photos)

    Matthew Morrison Engaged To Model Renee Puente (Photos)

    Matthew Morrison & Renee Puente pictures“Glee” star Matthew Morrison, 34, is engaged to his girlfriend of two years, model Renee Puente! Morrison and Puente’s engagement news was announced by Elton John and David Furnish at their annual White Tie And Tiara Ball in London on Thursday. Elton later serenaded Matthew and Renee when he performed a duet of “Your Song” ...

    Matthew Morrison Engaged To Model Renee Puente (Photos) Stupid Celebrities Gossip Stupid Celebrities Gossip News

    Source: http://stupidcelebrities.net/2013/06/matthew-morrison-engaged-to-model-renee-puente-photos/

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    Friday, June 28, 2013

    Motocross Fitness Routine - BenGreenfieldFitness.com

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    Wil Hahn (pictured above), a professional motocross racer, broke his hand in practice at the last round of the Supercross series, raced the main event with a broken hand, got 3rd place, then went on to won the overall title by a couple points.?

    Motocross is an extreme sport?

    ?outdoor motocross races occur in the heat of the day at for 30 minutes plus 2 laps, riding in full gear at lactate threshold for the entire time?

    ??armpump? can be a serious, painful problem when racing?

    ?dangerous levels of dehydration are extremely common?

    ?and in this BenGreenfieldFitness phone app insider interview with Wil, you?ll find out what kind of fitness and nutrition plan is necessary to succeed in one of the toughest sports on the face of the planet. Wil and I compare Ironman triathlon to motocross, talk about Wil?s motocross fitness routine, what Wil eats and drinks for motocross, tracking heart rates, racing with an injury, and more!

    Don?t believe that motocross is an extreme sport that ?just might beat up your body more than any other sport??Then watch this?crazy crash sequence video with Wil Hahn, and then keep reading for a motocross fitness routine?

    An Extreme Fitness Routine

    Motocross requires a combination of coordination, strength, power and extreme lactic acid buffering capabilities. To build this kind of extreme fitness, try?the following full body exercise routine, performed three times per week.

    -5-10 minute dynamic warm-up (Don?t know what a dynamic warm-up is? Read this mobility article.)

    -3-4 sets of 6-10 reps of each of the following, performed as either a circuit for an extreme twist, or with 60 seconds to 2 minutes recovery after each exercise for a more traditional approach (get exercise videos at YouTube.com/BenGreenfieldFitness)

    • Vertical Pulling (i.e. pull-up)
    • Vertical Pushing (i.e. overhead press)
    • Horizontal Pulling (i.e. seated row)
    • Horizontal Pushing (i.e. incline bench press)
    • Double or Single Leg Strength (i.e. squat)
    • Lift (i.e. deadlift)

    3-4 sets of 6-10 reps of any or all of the following, performed as either a circuit, or with 60 seconds to 2 minutes recovery after each exercise:

    • Slams (i.e. medicine ball slams)
    • Throws (i.e. medicine ball throws)
    • Tosses (i.e. medicine ball underhand throws)
    • Jumps (i.e. double leg box jumps)

    Finish with 3 sets of 12-15 reps, back-to-back, of each of the following:

    • Twists (i.e. cable torso twists)
    • Core flexion (i.e. hanging leg raises)

    Then, at a separate time of day, or on your ?non-lifting? day, do 20-30 minutes of high intensity interval training, which would include treadmill or cycling intervals, rowing, swimming, sprint repeats etc., preferably with time lengths of 20-60 seconds and rest intervals of 10-30 seconds.

    Assuming you?re also practicing or playing a sport, this routine is more than enough to get you fit fast.

    If you?re not practicing or playing a sport, you may want to add in a 2-4 extra 30-60 minute ?tempo? or aerobic workouts.

    After you take a listen to the interview (using the free?BenGreenfieldFitness phone app), or after you try the motocross fitness routine, leave your questions, comments and feedback below.

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    Source: http://www.bengreenfieldfitness.com/2013/06/motocross-fitness-routine/?utm_source=rss&utm_medium=rss&utm_campaign=motocross-fitness-routine

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    Source: http://dublingeorgia.blogspot.com/2013/06/tractor-supply-co-store-dublin-georgia.html

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    Starbucks reports UK loss, pays no tax for 2012

    By Tom Bergin

    LONDON (Reuters) - Starbucks, whose thin tax payments in Britain provoked a backlash against corporate tax avoidance when revealed by Reuters, paid no tax for the year to September 30, 2012.

    The coffee giant's main UK subsidiary reported its 15th straight annual loss at its UK stores in accounts filed on Friday.

    Reuters revealed in October that Starbucks reported consistent UK losses while telling investors the British unit was profitable and promoting managers of the unit within the group.

    Friday's accounts showed a UK loss of 30 million pounds ($46 million), down from the 32 million pounds loss it reported for the previous year, helped by a 4 percent rise in turnover to 413 million pounds.

    The company cited challenging economic conditions and a competitive UK coffee market, although the accounts show profits were also undermined by a royalty for the use of the Starbucks brand of 26 million pounds.

    This is paid to an affiliate in the Netherlands, where Starbucks has negotiated what it said was a very low tax rate.

    The UK unit also paid 2 million pounds in interest to affiliated companies, the accounts showed.

    Following widespread criticism from politicians and the picketing of stores, Starbucks said it would pay or pre-pay around 10 million pounds a year in taxes in 2013 and 2014.

    The company said it would not take tax deductions for certain intercompany payments such as the royalty fee, interest payments and the 25 percent mark up on coffee beans that is paid to a Swiss-based Starbucks coffee purchasing unit.

    Starbucks recently paid 5 million pounds to the UK tax authority as the first installment of its 2013 tax bill, British media reported earlier this month.

    Starbucks declined immediate comment.

    ($1 = 0.6593 British pounds)

    (Reporting by Tom Bergin; Editing by Ruth Pitchford)

    Source: http://news.yahoo.com/starbucks-reports-uk-loss-pays-no-tax-2012-182721959.html

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    Senate passes sweeping immigration legislation (reuters)

    Share With Friends: Share on FacebookTweet ThisPost to Google-BuzzSend on GmailPost to Linked-InSubscribe to This Feed | Rss To Twitter | Politics - Top Stories Stories, RSS Feeds and Widgets via Feedzilla.

    Source: http://news.feedzilla.com/en_us/stories/politics/top-stories/315744638?client_source=feed&format=rss

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    Hold the medicinal lettuce

    June 26, 2013 ? In 2011 and 2012, research from China's Nanjing University made international headlines with reports that after mice ate, bits of genetic material from the plants they'd ingested could make it into their bloodstreams intact and turn the animals' own genes off. The surprising results from Chen-Yu Zhang's group led to speculation that genetic illness might one day be treated with medicinal food, but also to worry that genetically modified foods might in turn modify consumers in unanticipated ways.

    Now, though, a research team at Johns Hopkins reports that Zhang's results were likely a false positive that resulted from the technique his group used. The new study, the Johns Hopkins group says, bolsters the case of skeptics who argued that genetic material from food would have little chance of surviving the digestive system, much less crossing the intestinal lining to enter the bloodstream. The study appears in the July issue of RNA Biology.

    "It's disappointing in a sense -- it would open up so many therapeutic possibilities if microRNAs from food really could get into our blood and regulate our genes," says Kenneth Witwer, Ph.D., of the Johns Hopkins University School of Medicine's Institute for Basic Biomedical Sciences, who led the new study. But beyond the fact that people won't be picking up prescription lettuce at the pharmacy anytime soon, he adds, the larger lesson is that scientific research's capacity for self-correction is alive and well.

    Witwer said his group was intrigued by the earlier results, in which Zhang's group focused on microRNAs, molecules that are a chemical cousin of DNA. Rather than storing genetic information as DNA does, their primary role is to intervene in so-called "gene expression," the process of using genes' blueprints to build proteins. Because they affect whether and how much genes are actually used, microRNAs wield tremendous power, Witwer notes, "so it was startling to think that microRNAs from plants could get into the bloodstream, get into tissues, and regulate genes in those tissues."

    Witwer teamed up with colleagues to check the results with a similar experiment of their own. They bought soy-based smoothies at a grocery store and tested their microRNA content, then fed the smoothies to macaques and took samples of the animals' blood.

    Knowing that the concentrations of any plant microRNAs in the blood would be too low to measure directly, they used a common technique called polymerase chain reaction (PCR) to bring up the concentration of the genetic material. PCR is designed so that only certain fragments of genetic material in a sample -- the ones researchers choose to target -- will be copied. Zhang's studies had also used PCR to look for plant microRNAs.

    Just as Zhang had, the Johns Hopkins team found what appeared to be the targeted plant microRNAs in the macaques' blood. But when they ran the experiment several times, they got highly variable results: Sometimes the microRNAs were present in low concentrations, and sometimes not at all. In addition, the samples from before the macaques drank the smoothies were just as likely to have the microRNAs as were the post-smoothie samples -- a result that just didn't make sense if the source of the microRNAs was the plant material in the drinks.

    To Witwer, the results indicated that what he was seeing was not the targeted plant microRNAs, but fragments of the macaques' own genetic material that were similar enough to the targeted segments that the PCR copied them at low levels.

    To test this, the team used a new technique in which PCR takes place in tiny aerosolized droplets rather than in a test tube. The advantage, Witwer says, is that by effectively running tens or hundreds of thousands of reactions at the same time, researchers can see whether the outcomes of those reactions are consistent -- in other words, whether the results are meaningful or just a fluke. In this case, the results were all over the place, indicating that plant microRNAs weren't really present.

    At the same time, Witwer cautions, it remains possible that very low levels of microRNAs could enter the blood. Even if this happened, though, he says it is unlikely that such small numbers of molecules could affect gene expression. Additional studies will be needed to determine whether low-level transfer occurs and whether any plant RNAs serve a function in the body.

    Source: http://feeds.sciencedaily.com/~r/sciencedaily/strange_science/~3/bwCVd6ZJxKk/130626183932.htm

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